An electrophoretic study of diphtheria toxoid preparations
نویسندگان
چکیده
منابع مشابه
An Electrophoretic Study of Immune Sera and Purified Antibody Preparations
1. Antibody produced in the horse migrates as a new serum component between the beta and gamma components, whereas rabbit antibody is electrophoretically identical with the gamma globulin component of the serum. 2. In rabbit and monkey antisera the percentage of antibody in the serum and in the gamma globulin fraction can be determined by integration of the electrophoresis diagrams of unabsorbe...
متن کاملPreparation and Evaluation of Diphtheria Toxoid-Containing Microspheres
Preparation of chitosan (CS) microspheres as a novel drug delivery vehicle for intranasal immunization using high, medium and low CS molecular weight (MW) was investigated in this study. Diphtheria toxoid (DT) was used as a model antigen. The emulsion-solidification method was adopted for microencapsulation of DT. In the first step, following the purification of semi-crude DT by the ion-exchan...
متن کاملThe Purification and Concentration of Diphtheria Toxoid
By precipitation with acetone at 4 degrees C. a refined diphtheria toxoid was obtained as a dry powder, readily soluble in aqueous solutions. The powder itself appears to be stable. When dissolved in half the original volume of physiological salt solution, the toxoid remained stable, in the cold room, for a period of 7 months. Only toxoids should be used which have been completely detoxified by...
متن کاملPreparation and Evaluation of Diphtheria Toxoid-Containing Microspheres
Preparation of chitosan (CS) microspheres as a novel drug delivery vehicle for intranasal immunization using high, medium and low CS molecular weight (MW) was investigated in this study. Diphtheria toxoid (DT) was used as a model antigen. The emulsion-solidification method was adopted for microencapsulation of DT. In the first step, following the purification of semi-crude DT by the ion-exchan...
متن کاملOPTIMIZATION OF DIPHTHERIA TOXOID PRECIPITATION BY USING SATURATED AMMONIUM SULPHATE IN ORDER TO IMPROVE DIPHTHERIA VACCINE
سابقه و هدف: کورینه باکتریوم دیفتریه باکتری هوازی و بیهوازی اختیاری است. مهمترین گونه از خانواده کورینه باکتریاها، عامل بیماری دیفتری است که به وسیله توکسین خود، بیماری دیفتری را ایجاد میکند. آمونیم سولفات نمکی معدنی است که از آن برای ترسیب و خالصسازی پروتئین ها استفاده میشود که با افزایش مقدار نمک حلالیت پروتئینی کم شده و پروتئین ها به واسطه قطعه های هیدروفوب خود، بههم پیوسته و رسو...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1948
ISSN: 0306-3283
DOI: 10.1042/bj0420425